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目前用于炎症性肠病诊断的肠镜和活检等技术存在侵入性，且难以进行定量评估和准确诊断，为此提出一种非介入、快速、易操作的微流控免疫传感器，将微流控富集技术和界面电容传感机制相结合，通过检测血清中的肠脂肪酸结合蛋白浓度，以无创方式进行炎症性肠病的诊断。实验结果表明，传感器可在30 s内测定血清中的目标蛋白含量，在标准品溶液中的检测限低至5.29×10-4 pg/mL,量程内的响应曲线呈半对数线性关系，选择比为8 130∶1。将传感器应用于临床受试者血清样本的检测，并与酶联免疫吸附测定(ELISA)法进行比较，两者检测结果的平均差异率为5.01%。本文提出的传感器为炎症性肠病的血清学诊断提供了有效解决方案，具有较高的无创诊断应用价值。
Abstract：At present, the diagnosis of inflammatory bowel disease(IBD) mainly relies on interventional techniques such as colonoscopy and biopsy, which make it difficult to realize quantitative assessment and accurate diagnosis.A non-invasive, fast, and easy-to-operate microfluidic immunosensor is developed.Combining microfluidic enrichment with interfacial capacitive sensing, the intestinal fatty acid binding proteins in serum are detected for non-invasive diagnosis of IBD.The experimental results show that the sensor can detect the target protein concentration in serum within 30 s, with a detection limit as low as 5.29×10-4 pg/mL in standard solutions.The response within the detection range is semi-logarithmic linear, with a selectivity of 8 130∶1 against the interference.The clinical deviation in serums is only 5.01% between the results from the sensor and those from the enzyme-linked immunosorbent assay(ELISA).The developed sensor provides an effective solution for serological diagnosis of IBD and has high noninvasive diagnostic application value.
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基本信息:
中图分类号:R574;TP212.3
引用信息:
[1]王天麒,张鉴,戚昊琛,等.用于炎症性肠病诊断的微流控界面电容式生物传感器[J].沈阳理工大学学报,2025,44(06):1-6.
基金信息:
国家自然科学基金项目(62074047); 浙江省自然科学基金项目(LQ24F040004)